QuickChek SRB
Introduction
QuickChekTM SRB offers several advantages over cell culture techniques for SRB detection including immediate and accurate results. The kit allows testing of solid and semi-solid samples and detects all SRB, including SRB that is unable to grow in some standard media. The test results are not compromised by chemical or salinity interferences that are often found in field samples.
The QuickChekTM kit is completely self-contained and disposable. Each kit includes all necessary materials to detect and enumerate sulfate reducing bacteria. There is no need for pretreatment or dilution of samples and there are not special hazardous waste disposal limitations..
Detection Principle
The QuickChekTM SRB Detection System is a rapid enzyme immunoassay method that detects sulfate reducing bacteria (SRB). The test employs purified antibodies to detect the enzyme adenosine-5’-phosphosulfonate (APS) reductase which is common to all strains of SRB.
The purified antibodies are attached to small particles that selectively capture the APS reductase enzyme. The particles and captured enzyme are then isolated on a porous membrane, which forms a reactive layer that turns blue in the presence of the APS reductase enzyme.
Features
- Semi-quantitative results
- Detects all viable and nonviable SRB in samples
- Results available in approximately 8-10 minutes
- Two or three samples simultaneously
- Detection limit of 10^3 cells/mL
- Detection limit for “clean” waters down to 10^1 cells/mL
Application
- Corrosion coupons
- Cutting fluids
- Drilling fluids (Muds)
- Solids: biofilms, sludge, rust, mud
- Water associated with oil